Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61649 |
| Pairs without alignments under any condition |
22150 |
| Pairs that did not map uniquely |
16201 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2190236 |
| Methylated C's in CpG context |
28825 |
| Methylated C's in CHG context |
1891 |
| Methylated C's in CHH context |
7592 |
| Methylated C's in Unknown context |
55 |
| Unmethylated C's in CpG context |
257120 |
| Unmethylated C's in CHG context |
381906 |
| Unmethylated C's in CHH context |
1512902 |
| Unmethylated C's in Unknown context |
5309 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15254 |
original top strand |
| CTOT |
15516 |
complementary to original top strand |
| CTOB |
15438 |
complementary to original bottom strand |
| OB |
15440 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61648 |
| Unique alignments |
57621 |
| Duplicates removed |
4027 |
| Duplicated alignments were found at 3762 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1404024 |
| Methylated C's in CpG context |
18040 |
| Methylated C's in CHG context |
1193 |
| Methylated C's in CHH context |
4894 |
| Unmethylated C's in CpG context |
163600 |
| Unmethylated C's in CHG context |
242229 |
| Unmethylated C's in CHH context |
974068 |
| Percentage methylation (CpG context) |
9.9% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot