Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_22_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_22_R2.fq.gz

Data processed at 23:10:14 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61649
Pairs without alignments under any condition 22150
Pairs that did not map uniquely 16201
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2190236
Methylated C's in CpG context 28825
Methylated C's in CHG context 1891
Methylated C's in CHH context 7592
Methylated C's in Unknown context 55
Unmethylated C's in CpG context 257120
Unmethylated C's in CHG context 381906
Unmethylated C's in CHH context 1512902
Unmethylated C's in Unknown context 5309
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15254 original top strand
CTOT 15516 complementary to original top strand
CTOB 15438 complementary to original bottom strand
OB 15440 original bottom strand

Deduplication

Alignments analysed 61648
Unique alignments 57621
Duplicates removed 4027
Duplicated alignments were found at 3762 different positions

Cytosine Methylation after Extraction

Total C's analysed 1404024
Methylated C's in CpG context 18040
Methylated C's in CHG context 1193
Methylated C's in CHH context 4894
Unmethylated C's in CpG context 163600
Unmethylated C's in CHG context 242229
Unmethylated C's in CHH context 974068
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot