Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_21_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_21_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61032
Pairs without alignments under any condition 22878
Pairs that did not map uniquely 16090
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2159763
Methylated C's in CpG context 29050
Methylated C's in CHG context 1750
Methylated C's in CHH context 7297
Methylated C's in Unknown context 71
Unmethylated C's in CpG context 250564
Unmethylated C's in CHG context 375069
Unmethylated C's in CHH context 1496033
Unmethylated C's in Unknown context 5010
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15175 original top strand
CTOT 15248 complementary to original top strand
CTOB 15358 complementary to original bottom strand
OB 15251 original bottom strand

Deduplication

Alignments analysed 61032
Unique alignments 56890
Duplicates removed 4142
Duplicated alignments were found at 3865 different positions

Cytosine Methylation after Extraction

Total C's analysed 1398119
Methylated C's in CpG context 18258
Methylated C's in CHG context 1122
Methylated C's in CHH context 4630
Unmethylated C's in CpG context 160954
Unmethylated C's in CHG context 240595
Unmethylated C's in CHH context 972560
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot