Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61032 |
| Pairs without alignments under any condition |
22878 |
| Pairs that did not map uniquely |
16090 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2159763 |
| Methylated C's in CpG context |
29050 |
| Methylated C's in CHG context |
1750 |
| Methylated C's in CHH context |
7297 |
| Methylated C's in Unknown context |
71 |
| Unmethylated C's in CpG context |
250564 |
| Unmethylated C's in CHG context |
375069 |
| Unmethylated C's in CHH context |
1496033 |
| Unmethylated C's in Unknown context |
5010 |
| Percentage methylation (CpG context) |
10.4% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15175 |
original top strand |
| CTOT |
15248 |
complementary to original top strand |
| CTOB |
15358 |
complementary to original bottom strand |
| OB |
15251 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61032 |
| Unique alignments |
56890 |
| Duplicates removed |
4142 |
| Duplicated alignments were found at 3865 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1398119 |
| Methylated C's in CpG context |
18258 |
| Methylated C's in CHG context |
1122 |
| Methylated C's in CHH context |
4630 |
| Unmethylated C's in CpG context |
160954 |
| Unmethylated C's in CHG context |
240595 |
| Unmethylated C's in CHH context |
972560 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot