Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_20_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_20_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61434
Pairs without alignments under any condition 22558
Pairs that did not map uniquely 16008
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 2172545
Methylated C's in CpG context 28306
Methylated C's in CHG context 1779
Methylated C's in CHH context 7639
Methylated C's in Unknown context 81
Unmethylated C's in CpG context 253674
Unmethylated C's in CHG context 376473
Unmethylated C's in CHH context 1504674
Unmethylated C's in Unknown context 5214
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14902 original top strand
CTOT 15777 complementary to original top strand
CTOB 15781 complementary to original bottom strand
OB 14972 original bottom strand

Deduplication

Alignments analysed 61432
Unique alignments 57268
Duplicates removed 4164
Duplicated alignments were found at 3878 different positions

Cytosine Methylation after Extraction

Total C's analysed 1406127
Methylated C's in CpG context 17661
Methylated C's in CHG context 1145
Methylated C's in CHH context 4861
Unmethylated C's in CpG context 163291
Unmethylated C's in CHG context 241742
Unmethylated C's in CHH context 977427
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot