Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61434 |
| Pairs without alignments under any condition |
22558 |
| Pairs that did not map uniquely |
16008 |
| Genomic sequence context not extractable (edges of chromosomes) |
2 |
|
|
Cytosine Methylation
| Total C's analysed |
2172545 |
| Methylated C's in CpG context |
28306 |
| Methylated C's in CHG context |
1779 |
| Methylated C's in CHH context |
7639 |
| Methylated C's in Unknown context |
81 |
| Unmethylated C's in CpG context |
253674 |
| Unmethylated C's in CHG context |
376473 |
| Unmethylated C's in CHH context |
1504674 |
| Unmethylated C's in Unknown context |
5214 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
14902 |
original top strand |
| CTOT |
15777 |
complementary to original top strand |
| CTOB |
15781 |
complementary to original bottom strand |
| OB |
14972 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61432 |
| Unique alignments |
57268 |
| Duplicates removed |
4164 |
| Duplicated alignments were found at 3878 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1406127 |
| Methylated C's in CpG context |
17661 |
| Methylated C's in CHG context |
1145 |
| Methylated C's in CHH context |
4861 |
| Unmethylated C's in CpG context |
163291 |
| Unmethylated C's in CHG context |
241742 |
| Unmethylated C's in CHH context |
977427 |
| Percentage methylation (CpG context) |
9.8% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot