Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_1_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_1_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 62198
Pairs without alignments under any condition 21145
Pairs that did not map uniquely 16657
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2196915
Methylated C's in CpG context 26894
Methylated C's in CHG context 1757
Methylated C's in CHH context 6890
Methylated C's in Unknown context 64
Unmethylated C's in CpG context 260090
Unmethylated C's in CHG context 385093
Unmethylated C's in CHH context 1516191
Unmethylated C's in Unknown context 5477
Percentage methylation (CpG context) 9.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15499 original top strand
CTOT 15602 complementary to original top strand
CTOB 15419 complementary to original bottom strand
OB 15678 original bottom strand

Deduplication

Alignments analysed 62198
Unique alignments 58103
Duplicates removed 4095
Duplicated alignments were found at 3834 different positions

Cytosine Methylation after Extraction

Total C's analysed 1376985
Methylated C's in CpG context 16501
Methylated C's in CHG context 1093
Methylated C's in CHH context 4349
Unmethylated C's in CpG context 161661
Unmethylated C's in CHG context 239101
Unmethylated C's in CHH context 954280
Percentage methylation (CpG context) 9.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot