Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_19_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_19_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61076
Pairs without alignments under any condition 22233
Pairs that did not map uniquely 16691
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2148194
Methylated C's in CpG context 27784
Methylated C's in CHG context 1793
Methylated C's in CHH context 7536
Methylated C's in Unknown context 53
Unmethylated C's in CpG context 250625
Unmethylated C's in CHG context 372639
Unmethylated C's in CHH context 1487817
Unmethylated C's in Unknown context 5360
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14959 original top strand
CTOT 15367 complementary to original top strand
CTOB 15363 complementary to original bottom strand
OB 15387 original bottom strand

Deduplication

Alignments analysed 61076
Unique alignments 57474
Duplicates removed 3602
Duplicated alignments were found at 3417 different positions

Cytosine Methylation after Extraction

Total C's analysed 1391794
Methylated C's in CpG context 17618
Methylated C's in CHG context 1166
Methylated C's in CHH context 4835
Unmethylated C's in CpG context 160667
Unmethylated C's in CHG context 239818
Unmethylated C's in CHH context 967690
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot