Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_18_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_18_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 62205
Pairs without alignments under any condition 21452
Pairs that did not map uniquely 16343
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2196700
Methylated C's in CpG context 29864
Methylated C's in CHG context 1818
Methylated C's in CHH context 7786
Methylated C's in Unknown context 71
Unmethylated C's in CpG context 258905
Unmethylated C's in CHG context 385573
Unmethylated C's in CHH context 1512754
Unmethylated C's in Unknown context 5230
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15450 original top strand
CTOT 15675 complementary to original top strand
CTOB 15522 complementary to original bottom strand
OB 15558 original bottom strand

Deduplication

Alignments analysed 62205
Unique alignments 58310
Duplicates removed 3895
Duplicated alignments were found at 3609 different positions

Cytosine Methylation after Extraction

Total C's analysed 1404454
Methylated C's in CpG context 18596
Methylated C's in CHG context 1178
Methylated C's in CHH context 4934
Unmethylated C's in CpG context 163930
Unmethylated C's in CHG context 244043
Unmethylated C's in CHH context 971773
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot