Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
62205 |
| Pairs without alignments under any condition |
21452 |
| Pairs that did not map uniquely |
16343 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2196700 |
| Methylated C's in CpG context |
29864 |
| Methylated C's in CHG context |
1818 |
| Methylated C's in CHH context |
7786 |
| Methylated C's in Unknown context |
71 |
| Unmethylated C's in CpG context |
258905 |
| Unmethylated C's in CHG context |
385573 |
| Unmethylated C's in CHH context |
1512754 |
| Unmethylated C's in Unknown context |
5230 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15450 |
original top strand |
| CTOT |
15675 |
complementary to original top strand |
| CTOB |
15522 |
complementary to original bottom strand |
| OB |
15558 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
62205 |
| Unique alignments |
58310 |
| Duplicates removed |
3895 |
| Duplicated alignments were found at 3609 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1404454 |
| Methylated C's in CpG context |
18596 |
| Methylated C's in CHG context |
1178 |
| Methylated C's in CHH context |
4934 |
| Unmethylated C's in CpG context |
163930 |
| Unmethylated C's in CHG context |
244043 |
| Unmethylated C's in CHH context |
971773 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot