Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61619 |
| Pairs without alignments under any condition |
21382 |
| Pairs that did not map uniquely |
16999 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2189226 |
| Methylated C's in CpG context |
29704 |
| Methylated C's in CHG context |
1910 |
| Methylated C's in CHH context |
7729 |
| Methylated C's in Unknown context |
73 |
| Unmethylated C's in CpG context |
261477 |
| Unmethylated C's in CHG context |
384145 |
| Unmethylated C's in CHH context |
1504261 |
| Unmethylated C's in Unknown context |
5167 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15152 |
original top strand |
| CTOT |
15598 |
complementary to original top strand |
| CTOB |
15386 |
complementary to original bottom strand |
| OB |
15483 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61619 |
| Unique alignments |
57253 |
| Duplicates removed |
4366 |
| Duplicated alignments were found at 4052 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1386305 |
| Methylated C's in CpG context |
18302 |
| Methylated C's in CHG context |
1206 |
| Methylated C's in CHH context |
4800 |
| Unmethylated C's in CpG context |
164021 |
| Unmethylated C's in CHG context |
241401 |
| Unmethylated C's in CHH context |
956575 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot