Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_17_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_17_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61619
Pairs without alignments under any condition 21382
Pairs that did not map uniquely 16999
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2189226
Methylated C's in CpG context 29704
Methylated C's in CHG context 1910
Methylated C's in CHH context 7729
Methylated C's in Unknown context 73
Unmethylated C's in CpG context 261477
Unmethylated C's in CHG context 384145
Unmethylated C's in CHH context 1504261
Unmethylated C's in Unknown context 5167
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15152 original top strand
CTOT 15598 complementary to original top strand
CTOB 15386 complementary to original bottom strand
OB 15483 original bottom strand

Deduplication

Alignments analysed 61619
Unique alignments 57253
Duplicates removed 4366
Duplicated alignments were found at 4052 different positions

Cytosine Methylation after Extraction

Total C's analysed 1386305
Methylated C's in CpG context 18302
Methylated C's in CHG context 1206
Methylated C's in CHH context 4800
Unmethylated C's in CpG context 164021
Unmethylated C's in CHG context 241401
Unmethylated C's in CHH context 956575
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot