Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_16_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_16_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61417
Pairs without alignments under any condition 21411
Pairs that did not map uniquely 17172
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2145277
Methylated C's in CpG context 29412
Methylated C's in CHG context 1874
Methylated C's in CHH context 7534
Methylated C's in Unknown context 72
Unmethylated C's in CpG context 251689
Unmethylated C's in CHG context 374150
Unmethylated C's in CHH context 1480618
Unmethylated C's in Unknown context 5061
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15314 original top strand
CTOT 15456 complementary to original top strand
CTOB 15634 complementary to original bottom strand
OB 15013 original bottom strand

Deduplication

Alignments analysed 61417
Unique alignments 57099
Duplicates removed 4318
Duplicated alignments were found at 4050 different positions

Cytosine Methylation after Extraction

Total C's analysed 1317943
Methylated C's in CpG context 17557
Methylated C's in CHG context 1110
Methylated C's in CHH context 4582
Unmethylated C's in CpG context 153340
Unmethylated C's in CHG context 227936
Unmethylated C's in CHH context 913418
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot