Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61239 |
| Pairs without alignments under any condition |
22623 |
| Pairs that did not map uniquely |
16138 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2167128 |
| Methylated C's in CpG context |
31015 |
| Methylated C's in CHG context |
1869 |
| Methylated C's in CHH context |
7405 |
| Methylated C's in Unknown context |
79 |
| Unmethylated C's in CpG context |
248991 |
| Unmethylated C's in CHG context |
374914 |
| Unmethylated C's in CHH context |
1502934 |
| Unmethylated C's in Unknown context |
5256 |
| Percentage methylation (CpG context) |
11.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15050 |
original top strand |
| CTOT |
15411 |
complementary to original top strand |
| CTOB |
15393 |
complementary to original bottom strand |
| OB |
15385 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61239 |
| Unique alignments |
57288 |
| Duplicates removed |
3951 |
| Duplicated alignments were found at 3740 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1389611 |
| Methylated C's in CpG context |
19351 |
| Methylated C's in CHG context |
1181 |
| Methylated C's in CHH context |
4711 |
| Unmethylated C's in CpG context |
158139 |
| Unmethylated C's in CHG context |
238682 |
| Unmethylated C's in CHH context |
967547 |
| Percentage methylation (CpG context) |
10.9% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot