Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_15_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_15_R2.fq.gz

Data processed at 23:10:13 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61239
Pairs without alignments under any condition 22623
Pairs that did not map uniquely 16138
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2167128
Methylated C's in CpG context 31015
Methylated C's in CHG context 1869
Methylated C's in CHH context 7405
Methylated C's in Unknown context 79
Unmethylated C's in CpG context 248991
Unmethylated C's in CHG context 374914
Unmethylated C's in CHH context 1502934
Unmethylated C's in Unknown context 5256
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15050 original top strand
CTOT 15411 complementary to original top strand
CTOB 15393 complementary to original bottom strand
OB 15385 original bottom strand

Deduplication

Alignments analysed 61239
Unique alignments 57288
Duplicates removed 3951
Duplicated alignments were found at 3740 different positions

Cytosine Methylation after Extraction

Total C's analysed 1389611
Methylated C's in CpG context 19351
Methylated C's in CHG context 1181
Methylated C's in CHH context 4711
Unmethylated C's in CpG context 158139
Unmethylated C's in CHG context 238682
Unmethylated C's in CHH context 967547
Percentage methylation (CpG context) 10.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot