Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
61532 |
Pairs without alignments under any condition |
22553 |
Pairs that did not map uniquely |
15915 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2182732 |
Methylated C's in CpG context |
28969 |
Methylated C's in CHG context |
1775 |
Methylated C's in CHH context |
7465 |
Methylated C's in Unknown context |
87 |
Unmethylated C's in CpG context |
252396 |
Unmethylated C's in CHG context |
378860 |
Unmethylated C's in CHH context |
1513267 |
Unmethylated C's in Unknown context |
5358 |
Percentage methylation (CpG context) |
10.3% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
15387 |
original top strand |
CTOT |
15376 |
complementary to original top strand |
CTOB |
15360 |
complementary to original bottom strand |
OB |
15409 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
61532 |
Unique alignments |
57093 |
Duplicates removed |
4439 |
Duplicated alignments were found at 4149 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1407110 |
Methylated C's in CpG context |
18235 |
Methylated C's in CHG context |
1159 |
Methylated C's in CHH context |
4777 |
Unmethylated C's in CpG context |
161556 |
Unmethylated C's in CHG context |
241902 |
Unmethylated C's in CHH context |
979481 |
Percentage methylation (CpG context) |
10.1% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot