Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_12_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_12_R2.fq.gz

Data processed at 23:10:12 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61267
Pairs without alignments under any condition 22727
Pairs that did not map uniquely 16006
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2136244
Methylated C's in CpG context 26364
Methylated C's in CHG context 1662
Methylated C's in CHH context 7158
Methylated C's in Unknown context 63
Unmethylated C's in CpG context 242814
Unmethylated C's in CHG context 360190
Unmethylated C's in CHH context 1498056
Unmethylated C's in Unknown context 5263
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15337 original top strand
CTOT 15464 complementary to original top strand
CTOB 15341 complementary to original bottom strand
OB 15125 original bottom strand

Deduplication

Alignments analysed 61267
Unique alignments 57357
Duplicates removed 3910
Duplicated alignments were found at 3678 different positions

Cytosine Methylation after Extraction

Total C's analysed 1359316
Methylated C's in CpG context 16319
Methylated C's in CHG context 1018
Methylated C's in CHH context 4474
Unmethylated C's in CpG context 153209
Unmethylated C's in CHG context 226939
Unmethylated C's in CHH context 957357
Percentage methylation (CpG context) 9.6%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%

M-Bias Plot