Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61267 |
| Pairs without alignments under any condition |
22727 |
| Pairs that did not map uniquely |
16006 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2136244 |
| Methylated C's in CpG context |
26364 |
| Methylated C's in CHG context |
1662 |
| Methylated C's in CHH context |
7158 |
| Methylated C's in Unknown context |
63 |
| Unmethylated C's in CpG context |
242814 |
| Unmethylated C's in CHG context |
360190 |
| Unmethylated C's in CHH context |
1498056 |
| Unmethylated C's in Unknown context |
5263 |
| Percentage methylation (CpG context) |
9.8% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15337 |
original top strand |
| CTOT |
15464 |
complementary to original top strand |
| CTOB |
15341 |
complementary to original bottom strand |
| OB |
15125 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61267 |
| Unique alignments |
57357 |
| Duplicates removed |
3910 |
| Duplicated alignments were found at 3678 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1359316 |
| Methylated C's in CpG context |
16319 |
| Methylated C's in CHG context |
1018 |
| Methylated C's in CHH context |
4474 |
| Unmethylated C's in CpG context |
153209 |
| Unmethylated C's in CHG context |
226939 |
| Unmethylated C's in CHH context |
957357 |
| Percentage methylation (CpG context) |
9.6% |
| Percentage methylation (CHG context) |
0.4% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot