Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_11_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_11_R2.fq.gz

Data processed at 23:10:12 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60730
Pairs without alignments under any condition 22750
Pairs that did not map uniquely 16520
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2144414
Methylated C's in CpG context 28604
Methylated C's in CHG context 1743
Methylated C's in CHH context 7591
Methylated C's in Unknown context 74
Unmethylated C's in CpG context 251327
Unmethylated C's in CHG context 375324
Unmethylated C's in CHH context 1479825
Unmethylated C's in Unknown context 5350
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15196 original top strand
CTOT 15134 complementary to original top strand
CTOB 15334 complementary to original bottom strand
OB 15066 original bottom strand

Deduplication

Alignments analysed 60730
Unique alignments 56685
Duplicates removed 4045
Duplicated alignments were found at 3763 different positions

Cytosine Methylation after Extraction

Total C's analysed 1361369
Methylated C's in CpG context 17748
Methylated C's in CHG context 1093
Methylated C's in CHH context 4739
Unmethylated C's in CpG context 158161
Unmethylated C's in CHG context 235802
Unmethylated C's in CHH context 943826
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot