Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_10_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_10_R2.fq.gz

Data processed at 23:10:12 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60927
Pairs without alignments under any condition 22647
Pairs that did not map uniquely 16426
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2156193
Methylated C's in CpG context 29771
Methylated C's in CHG context 1786
Methylated C's in CHH context 7551
Methylated C's in Unknown context 68
Unmethylated C's in CpG context 252863
Unmethylated C's in CHG context 376521
Unmethylated C's in CHH context 1487701
Unmethylated C's in Unknown context 5259
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15178 original top strand
CTOT 15142 complementary to original top strand
CTOB 15261 complementary to original bottom strand
OB 15345 original bottom strand

Deduplication

Alignments analysed 60926
Unique alignments 56946
Duplicates removed 3980
Duplicated alignments were found at 3685 different positions

Cytosine Methylation after Extraction

Total C's analysed 1371132
Methylated C's in CpG context 18394
Methylated C's in CHG context 1125
Methylated C's in CHH context 4852
Unmethylated C's in CpG context 159797
Unmethylated C's in CHG context 237123
Unmethylated C's in CHH context 949841
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot