Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3616_8_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3616_8_R2.fq.gz

Data processed at 23:10:12 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61458
Pairs without alignments under any condition 18881
Pairs that did not map uniquely 19661
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2027999
Methylated C's in CpG context 30597
Methylated C's in CHG context 1889
Methylated C's in CHH context 8508
Methylated C's in Unknown context 101
Unmethylated C's in CpG context 247291
Unmethylated C's in CHG context 367521
Unmethylated C's in CHH context 1372193
Unmethylated C's in Unknown context 4708
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15324 original top strand
CTOT 15381 complementary to original top strand
CTOB 15359 complementary to original bottom strand
OB 15394 original bottom strand

Deduplication

Alignments analysed 61458
Unique alignments 53992
Duplicates removed 7466
Duplicated alignments were found at 6463 different positions

Cytosine Methylation after Extraction

Total C's analysed 1057836
Methylated C's in CpG context 15560
Methylated C's in CHG context 953
Methylated C's in CHH context 4356
Unmethylated C's in CpG context 128008
Unmethylated C's in CHG context 189631
Unmethylated C's in CHH context 719328
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot