Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3616_7_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3616_7_R2.fq.gz

Data processed at 23:10:12 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61555
Pairs without alignments under any condition 19307
Pairs that did not map uniquely 19138
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 1998027
Methylated C's in CpG context 30581
Methylated C's in CHG context 1797
Methylated C's in CHH context 7852
Methylated C's in Unknown context 80
Unmethylated C's in CpG context 239023
Unmethylated C's in CHG context 357714
Unmethylated C's in CHH context 1361060
Unmethylated C's in Unknown context 4895
Percentage methylation (CpG context) 11.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15617 original top strand
CTOT 15277 complementary to original top strand
CTOB 15198 complementary to original bottom strand
OB 15462 original bottom strand

Deduplication

Alignments analysed 61554
Unique alignments 53945
Duplicates removed 7609
Duplicated alignments were found at 6539 different positions

Cytosine Methylation after Extraction

Total C's analysed 1042333
Methylated C's in CpG context 15524
Methylated C's in CHG context 930
Methylated C's in CHH context 4069
Unmethylated C's in CpG context 123948
Unmethylated C's in CHG context 184748
Unmethylated C's in CHH context 713114
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot