Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61555 |
| Pairs without alignments under any condition |
19307 |
| Pairs that did not map uniquely |
19138 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
1998027 |
| Methylated C's in CpG context |
30581 |
| Methylated C's in CHG context |
1797 |
| Methylated C's in CHH context |
7852 |
| Methylated C's in Unknown context |
80 |
| Unmethylated C's in CpG context |
239023 |
| Unmethylated C's in CHG context |
357714 |
| Unmethylated C's in CHH context |
1361060 |
| Unmethylated C's in Unknown context |
4895 |
| Percentage methylation (CpG context) |
11.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15617 |
original top strand |
| CTOT |
15277 |
complementary to original top strand |
| CTOB |
15198 |
complementary to original bottom strand |
| OB |
15462 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61554 |
| Unique alignments |
53945 |
| Duplicates removed |
7609 |
| Duplicated alignments were found at 6539 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1042333 |
| Methylated C's in CpG context |
15524 |
| Methylated C's in CHG context |
930 |
| Methylated C's in CHH context |
4069 |
| Unmethylated C's in CpG context |
123948 |
| Unmethylated C's in CHG context |
184748 |
| Unmethylated C's in CHH context |
713114 |
| Percentage methylation (CpG context) |
11.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
|
|
M-Bias Plot