Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61682 |
| Pairs without alignments under any condition |
18693 |
| Pairs that did not map uniquely |
19625 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2012509 |
| Methylated C's in CpG context |
28226 |
| Methylated C's in CHG context |
1768 |
| Methylated C's in CHH context |
7979 |
| Methylated C's in Unknown context |
76 |
| Unmethylated C's in CpG context |
245141 |
| Unmethylated C's in CHG context |
362340 |
| Unmethylated C's in CHH context |
1367055 |
| Unmethylated C's in Unknown context |
4713 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15603 |
original top strand |
| CTOT |
15314 |
complementary to original top strand |
| CTOB |
15482 |
complementary to original bottom strand |
| OB |
15282 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61681 |
| Unique alignments |
54309 |
| Duplicates removed |
7372 |
| Duplicated alignments were found at 6394 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1037483 |
| Methylated C's in CpG context |
14188 |
| Methylated C's in CHG context |
898 |
| Methylated C's in CHH context |
4269 |
| Unmethylated C's in CpG context |
125402 |
| Unmethylated C's in CHG context |
185487 |
| Unmethylated C's in CHH context |
707239 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
|
|
M-Bias Plot