Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3616_4_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3616_4_R2.fq.gz

Data processed at 23:10:11 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61653
Pairs without alignments under any condition 18849
Pairs that did not map uniquely 19498
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2033622
Methylated C's in CpG context 29878
Methylated C's in CHG context 1920
Methylated C's in CHH context 7954
Methylated C's in Unknown context 72
Unmethylated C's in CpG context 247745
Unmethylated C's in CHG context 368893
Unmethylated C's in CHH context 1377232
Unmethylated C's in Unknown context 4794
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15417 original top strand
CTOT 15210 complementary to original top strand
CTOB 15469 complementary to original bottom strand
OB 15557 original bottom strand

Deduplication

Alignments analysed 61653
Unique alignments 53932
Duplicates removed 7721
Duplicated alignments were found at 6649 different positions

Cytosine Methylation after Extraction

Total C's analysed 1054411
Methylated C's in CpG context 15340
Methylated C's in CHG context 965
Methylated C's in CHH context 4152
Unmethylated C's in CpG context 127416
Unmethylated C's in CHG context 189571
Unmethylated C's in CHH context 716967
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot