Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3616_3_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3616_3_R2.fq.gz

Data processed at 23:10:11 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61506
Pairs without alignments under any condition 18728
Pairs that did not map uniquely 19766
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 1975265
Methylated C's in CpG context 29898
Methylated C's in CHG context 1808
Methylated C's in CHH context 7634
Methylated C's in Unknown context 63
Unmethylated C's in CpG context 236799
Unmethylated C's in CHG context 355129
Unmethylated C's in CHH context 1343997
Unmethylated C's in Unknown context 4788
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15466 original top strand
CTOT 15481 complementary to original top strand
CTOB 15358 complementary to original bottom strand
OB 15201 original bottom strand

Deduplication

Alignments analysed 61506
Unique alignments 53738
Duplicates removed 7768
Duplicated alignments were found at 6710 different positions

Cytosine Methylation after Extraction

Total C's analysed 999365
Methylated C's in CpG context 14715
Methylated C's in CHG context 901
Methylated C's in CHH context 3908
Unmethylated C's in CpG context 119091
Unmethylated C's in CHG context 177915
Unmethylated C's in CHH context 682835
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot