Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3616_2_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3616_2_R2.fq.gz

Data processed at 23:10:11 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61847
Pairs without alignments under any condition 19291
Pairs that did not map uniquely 18862
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2080161
Methylated C's in CpG context 30602
Methylated C's in CHG context 1923
Methylated C's in CHH context 7653
Methylated C's in Unknown context 76
Unmethylated C's in CpG context 255192
Unmethylated C's in CHG context 374869
Unmethylated C's in CHH context 1409922
Unmethylated C's in Unknown context 4821
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15384 original top strand
CTOT 15310 complementary to original top strand
CTOB 15418 complementary to original bottom strand
OB 15735 original bottom strand

Deduplication

Alignments analysed 61847
Unique alignments 54090
Duplicates removed 7757
Duplicated alignments were found at 6637 different positions

Cytosine Methylation after Extraction

Total C's analysed 1094017
Methylated C's in CpG context 15537
Methylated C's in CHG context 972
Methylated C's in CHH context 4051
Unmethylated C's in CpG context 133072
Unmethylated C's in CHG context 195204
Unmethylated C's in CHH context 745181
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot