Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3616_1_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3616_1_R2.fq.gz

Data processed at 23:10:11 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 62078
Pairs without alignments under any condition 18843
Pairs that did not map uniquely 19079
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2097641
Methylated C's in CpG context 31375
Methylated C's in CHG context 1898
Methylated C's in CHH context 8727
Methylated C's in Unknown context 90
Unmethylated C's in CpG context 258663
Unmethylated C's in CHG context 378675
Unmethylated C's in CHH context 1418303
Unmethylated C's in Unknown context 4928
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15615 original top strand
CTOT 15682 complementary to original top strand
CTOB 15245 complementary to original bottom strand
OB 15536 original bottom strand

Deduplication

Alignments analysed 62078
Unique alignments 54267
Duplicates removed 7811
Duplicated alignments were found at 6700 different positions

Cytosine Methylation after Extraction

Total C's analysed 1111357
Methylated C's in CpG context 16063
Methylated C's in CHG context 1008
Methylated C's in CHH context 4642
Unmethylated C's in CpG context 135628
Unmethylated C's in CHG context 198837
Unmethylated C's in CHH context 755179
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot