Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
62078 |
| Pairs without alignments under any condition |
18843 |
| Pairs that did not map uniquely |
19079 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2097641 |
| Methylated C's in CpG context |
31375 |
| Methylated C's in CHG context |
1898 |
| Methylated C's in CHH context |
8727 |
| Methylated C's in Unknown context |
90 |
| Unmethylated C's in CpG context |
258663 |
| Unmethylated C's in CHG context |
378675 |
| Unmethylated C's in CHH context |
1418303 |
| Unmethylated C's in Unknown context |
4928 |
| Percentage methylation (CpG context) |
10.8% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15615 |
original top strand |
| CTOT |
15682 |
complementary to original top strand |
| CTOB |
15245 |
complementary to original bottom strand |
| OB |
15536 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
62078 |
| Unique alignments |
54267 |
| Duplicates removed |
7811 |
| Duplicated alignments were found at 6700 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1111357 |
| Methylated C's in CpG context |
16063 |
| Methylated C's in CHG context |
1008 |
| Methylated C's in CHH context |
4642 |
| Unmethylated C's in CpG context |
135628 |
| Unmethylated C's in CHG context |
198837 |
| Unmethylated C's in CHH context |
755179 |
| Percentage methylation (CpG context) |
10.6% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
|
|
M-Bias Plot