Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61092 |
| Pairs without alignments under any condition |
20677 |
| Pairs that did not map uniquely |
18231 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2114911 |
| Methylated C's in CpG context |
29822 |
| Methylated C's in CHG context |
1724 |
| Methylated C's in CHH context |
7855 |
| Methylated C's in Unknown context |
76 |
| Unmethylated C's in CpG context |
245639 |
| Unmethylated C's in CHG context |
368266 |
| Unmethylated C's in CHH context |
1461605 |
| Unmethylated C's in Unknown context |
5097 |
| Percentage methylation (CpG context) |
10.8% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15406 |
original top strand |
| CTOT |
14990 |
complementary to original top strand |
| CTOB |
15383 |
complementary to original bottom strand |
| OB |
15313 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61092 |
| Unique alignments |
56719 |
| Duplicates removed |
4373 |
| Duplicated alignments were found at 4076 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1283697 |
| Methylated C's in CpG context |
17534 |
| Methylated C's in CHG context |
1072 |
| Methylated C's in CHH context |
4784 |
| Unmethylated C's in CpG context |
147837 |
| Unmethylated C's in CHG context |
221612 |
| Unmethylated C's in CHH context |
890858 |
| Percentage methylation (CpG context) |
10.6% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot