Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61250 |
| Pairs without alignments under any condition |
21911 |
| Pairs that did not map uniquely |
16839 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2157029 |
| Methylated C's in CpG context |
28381 |
| Methylated C's in CHG context |
1690 |
| Methylated C's in CHH context |
7022 |
| Methylated C's in Unknown context |
85 |
| Unmethylated C's in CpG context |
246166 |
| Unmethylated C's in CHG context |
369285 |
| Unmethylated C's in CHH context |
1504485 |
| Unmethylated C's in Unknown context |
5278 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15247 |
original top strand |
| CTOT |
15316 |
complementary to original top strand |
| CTOB |
15277 |
complementary to original bottom strand |
| OB |
15410 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61250 |
| Unique alignments |
57089 |
| Duplicates removed |
4161 |
| Duplicated alignments were found at 3910 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1371032 |
| Methylated C's in CpG context |
17454 |
| Methylated C's in CHG context |
1054 |
| Methylated C's in CHH context |
4473 |
| Unmethylated C's in CpG context |
155258 |
| Unmethylated C's in CHG context |
232556 |
| Unmethylated C's in CHH context |
960237 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot