Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_3_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_3_R2.fq.gz

Data processed at 23:10:10 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61161
Pairs without alignments under any condition 21806
Pairs that did not map uniquely 17033
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2154532
Methylated C's in CpG context 28478
Methylated C's in CHG context 1768
Methylated C's in CHH context 7387
Methylated C's in Unknown context 62
Unmethylated C's in CpG context 250025
Unmethylated C's in CHG context 372477
Unmethylated C's in CHH context 1494397
Unmethylated C's in Unknown context 5422
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15370 original top strand
CTOT 15366 complementary to original top strand
CTOB 15264 complementary to original bottom strand
OB 15161 original bottom strand

Deduplication

Alignments analysed 61161
Unique alignments 57491
Duplicates removed 3670
Duplicated alignments were found at 3431 different positions

Cytosine Methylation after Extraction

Total C's analysed 1386996
Methylated C's in CpG context 17858
Methylated C's in CHG context 1119
Methylated C's in CHH context 4720
Unmethylated C's in CpG context 160089
Unmethylated C's in CHG context 237922
Unmethylated C's in CHH context 965288
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot