Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
62315 |
| Pairs without alignments under any condition |
21022 |
| Pairs that did not map uniquely |
16663 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2207527 |
| Methylated C's in CpG context |
29017 |
| Methylated C's in CHG context |
1829 |
| Methylated C's in CHH context |
7614 |
| Methylated C's in Unknown context |
66 |
| Unmethylated C's in CpG context |
258161 |
| Unmethylated C's in CHG context |
383928 |
| Unmethylated C's in CHH context |
1526978 |
| Unmethylated C's in Unknown context |
5334 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15540 |
original top strand |
| CTOT |
15661 |
complementary to original top strand |
| CTOB |
15398 |
complementary to original bottom strand |
| OB |
15716 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
62315 |
| Unique alignments |
58133 |
| Duplicates removed |
4182 |
| Duplicated alignments were found at 3943 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1405268 |
| Methylated C's in CpG context |
18046 |
| Methylated C's in CHG context |
1163 |
| Methylated C's in CHH context |
4781 |
| Unmethylated C's in CpG context |
163678 |
| Unmethylated C's in CHG context |
242659 |
| Unmethylated C's in CHH context |
974941 |
| Percentage methylation (CpG context) |
9.9% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot