Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_23_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_23_R2.fq.gz

Data processed at 23:10:09 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61316
Pairs without alignments under any condition 21132
Pairs that did not map uniquely 17552
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2147258
Methylated C's in CpG context 28217
Methylated C's in CHG context 1894
Methylated C's in CHH context 7705
Methylated C's in Unknown context 40
Unmethylated C's in CpG context 251795
Unmethylated C's in CHG context 374551
Unmethylated C's in CHH context 1483096
Unmethylated C's in Unknown context 5236
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15304 original top strand
CTOT 15128 complementary to original top strand
CTOB 15103 complementary to original bottom strand
OB 15781 original bottom strand

Deduplication

Alignments analysed 61316
Unique alignments 57209
Duplicates removed 4107
Duplicated alignments were found at 3847 different positions

Cytosine Methylation after Extraction

Total C's analysed 1354323
Methylated C's in CpG context 17488
Methylated C's in CHG context 1183
Methylated C's in CHH context 4909
Unmethylated C's in CpG context 157505
Unmethylated C's in CHG context 233818
Unmethylated C's in CHH context 939420
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot