Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61355 |
| Pairs without alignments under any condition |
22075 |
| Pairs that did not map uniquely |
16570 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2163758 |
| Methylated C's in CpG context |
28956 |
| Methylated C's in CHG context |
1733 |
| Methylated C's in CHH context |
7474 |
| Methylated C's in Unknown context |
76 |
| Unmethylated C's in CpG context |
250171 |
| Unmethylated C's in CHG context |
375364 |
| Unmethylated C's in CHH context |
1500060 |
| Unmethylated C's in Unknown context |
5000 |
| Percentage methylation (CpG context) |
10.4% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15229 |
original top strand |
| CTOT |
15342 |
complementary to original top strand |
| CTOB |
15476 |
complementary to original bottom strand |
| OB |
15307 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61354 |
| Unique alignments |
57158 |
| Duplicates removed |
4196 |
| Duplicated alignments were found at 3916 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1396963 |
| Methylated C's in CpG context |
18229 |
| Methylated C's in CHG context |
1108 |
| Methylated C's in CHH context |
4713 |
| Unmethylated C's in CpG context |
160395 |
| Unmethylated C's in CHG context |
240305 |
| Unmethylated C's in CHH context |
972213 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot