Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_20_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_20_R2.fq.gz

Data processed at 23:10:09 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61594
Pairs without alignments under any condition 21962
Pairs that did not map uniquely 16444
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 2172583
Methylated C's in CpG context 28191
Methylated C's in CHG context 1786
Methylated C's in CHH context 8067
Methylated C's in Unknown context 91
Unmethylated C's in CpG context 252733
Unmethylated C's in CHG context 376351
Unmethylated C's in CHH context 1505455
Unmethylated C's in Unknown context 5247
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15039 original top strand
CTOT 15791 complementary to original top strand
CTOB 15787 complementary to original bottom strand
OB 14975 original bottom strand

Deduplication

Alignments analysed 61592
Unique alignments 57403
Duplicates removed 4189
Duplicated alignments were found at 3896 different positions

Cytosine Methylation after Extraction

Total C's analysed 1402572
Methylated C's in CpG context 17549
Methylated C's in CHG context 1151
Methylated C's in CHH context 5152
Unmethylated C's in CpG context 162343
Unmethylated C's in CHG context 241099
Unmethylated C's in CHH context 975278
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot