Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_19_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_19_R2.fq.gz

Data processed at 23:10:09 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61402
Pairs without alignments under any condition 21445
Pairs that did not map uniquely 17153
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2151706
Methylated C's in CpG context 27843
Methylated C's in CHG context 1773
Methylated C's in CHH context 7762
Methylated C's in Unknown context 53
Unmethylated C's in CpG context 250344
Unmethylated C's in CHG context 372609
Unmethylated C's in CHH context 1491375
Unmethylated C's in Unknown context 5466
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15058 original top strand
CTOT 15440 complementary to original top strand
CTOB 15449 complementary to original bottom strand
OB 15455 original bottom strand

Deduplication

Alignments analysed 61402
Unique alignments 57782
Duplicates removed 3620
Duplicated alignments were found at 3430 different positions

Cytosine Methylation after Extraction

Total C's analysed 1391498
Methylated C's in CpG context 17611
Methylated C's in CHG context 1159
Methylated C's in CHH context 5021
Unmethylated C's in CpG context 160205
Unmethylated C's in CHG context 239409
Unmethylated C's in CHH context 968093
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot