Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_18_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_18_R2.fq.gz

Data processed at 23:10:08 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 62397
Pairs without alignments under any condition 20763
Pairs that did not map uniquely 16840
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2194162
Methylated C's in CpG context 29865
Methylated C's in CHG context 1793
Methylated C's in CHH context 7718
Methylated C's in Unknown context 60
Unmethylated C's in CpG context 257540
Unmethylated C's in CHG context 384284
Unmethylated C's in CHH context 1512962
Unmethylated C's in Unknown context 5240
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15525 original top strand
CTOT 15680 complementary to original top strand
CTOB 15544 complementary to original bottom strand
OB 15648 original bottom strand

Deduplication

Alignments analysed 62397
Unique alignments 58506
Duplicates removed 3891
Duplicated alignments were found at 3610 different positions

Cytosine Methylation after Extraction

Total C's analysed 1400104
Methylated C's in CpG context 18495
Methylated C's in CHG context 1162
Methylated C's in CHH context 4926
Unmethylated C's in CpG context 162988
Unmethylated C's in CHG context 242924
Unmethylated C's in CHH context 969609
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot