Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
62397 |
Pairs without alignments under any condition |
20763 |
Pairs that did not map uniquely |
16840 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2194162 |
Methylated C's in CpG context |
29865 |
Methylated C's in CHG context |
1793 |
Methylated C's in CHH context |
7718 |
Methylated C's in Unknown context |
60 |
Unmethylated C's in CpG context |
257540 |
Unmethylated C's in CHG context |
384284 |
Unmethylated C's in CHH context |
1512962 |
Unmethylated C's in Unknown context |
5240 |
Percentage methylation (CpG context) |
10.4% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
15525 |
original top strand |
CTOT |
15680 |
complementary to original top strand |
CTOB |
15544 |
complementary to original bottom strand |
OB |
15648 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
62397 |
Unique alignments |
58506 |
Duplicates removed |
3891 |
Duplicated alignments were found at 3610 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1400104 |
Methylated C's in CpG context |
18495 |
Methylated C's in CHG context |
1162 |
Methylated C's in CHH context |
4926 |
Unmethylated C's in CpG context |
162988 |
Unmethylated C's in CHG context |
242924 |
Unmethylated C's in CHH context |
969609 |
Percentage methylation (CpG context) |
10.2% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot