Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61805 |
| Pairs without alignments under any condition |
20526 |
| Pairs that did not map uniquely |
17669 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2184728 |
| Methylated C's in CpG context |
29618 |
| Methylated C's in CHG context |
1881 |
| Methylated C's in CHH context |
7761 |
| Methylated C's in Unknown context |
75 |
| Unmethylated C's in CpG context |
260260 |
| Unmethylated C's in CHG context |
382957 |
| Unmethylated C's in CHH context |
1502251 |
| Unmethylated C's in Unknown context |
5205 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15182 |
original top strand |
| CTOT |
15650 |
complementary to original top strand |
| CTOB |
15428 |
complementary to original bottom strand |
| OB |
15545 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61805 |
| Unique alignments |
57402 |
| Duplicates removed |
4403 |
| Duplicated alignments were found at 4086 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1379883 |
| Methylated C's in CpG context |
18203 |
| Methylated C's in CHG context |
1177 |
| Methylated C's in CHH context |
4744 |
| Unmethylated C's in CpG context |
162900 |
| Unmethylated C's in CHG context |
239940 |
| Unmethylated C's in CHH context |
952919 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot