Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
62089 |
| Pairs without alignments under any condition |
21390 |
| Pairs that did not map uniquely |
16521 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2178029 |
| Methylated C's in CpG context |
32720 |
| Methylated C's in CHG context |
1899 |
| Methylated C's in CHH context |
7658 |
| Methylated C's in Unknown context |
85 |
| Unmethylated C's in CpG context |
246804 |
| Unmethylated C's in CHG context |
376144 |
| Unmethylated C's in CHH context |
1512804 |
| Unmethylated C's in Unknown context |
5234 |
| Percentage methylation (CpG context) |
11.7% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15451 |
original top strand |
| CTOT |
15505 |
complementary to original top strand |
| CTOB |
15794 |
complementary to original bottom strand |
| OB |
15338 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
62088 |
| Unique alignments |
58034 |
| Duplicates removed |
4054 |
| Duplicated alignments were found at 3786 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1392769 |
| Methylated C's in CpG context |
20439 |
| Methylated C's in CHG context |
1189 |
| Methylated C's in CHH context |
4943 |
| Unmethylated C's in CpG context |
156912 |
| Unmethylated C's in CHG context |
238595 |
| Unmethylated C's in CHH context |
970691 |
| Percentage methylation (CpG context) |
11.5% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot