Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
61792 |
Pairs without alignments under any condition |
21880 |
Pairs that did not map uniquely |
16328 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2186112 |
Methylated C's in CpG context |
28902 |
Methylated C's in CHG context |
1781 |
Methylated C's in CHH context |
7859 |
Methylated C's in Unknown context |
89 |
Unmethylated C's in CpG context |
251857 |
Unmethylated C's in CHG context |
378544 |
Unmethylated C's in CHH context |
1517169 |
Unmethylated C's in Unknown context |
5394 |
Percentage methylation (CpG context) |
10.3% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
15417 |
original top strand |
CTOT |
15456 |
complementary to original top strand |
CTOB |
15454 |
complementary to original bottom strand |
OB |
15465 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
61792 |
Unique alignments |
57342 |
Duplicates removed |
4450 |
Duplicated alignments were found at 4165 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1406264 |
Methylated C's in CpG context |
18175 |
Methylated C's in CHG context |
1149 |
Methylated C's in CHH context |
5038 |
Unmethylated C's in CpG context |
160953 |
Unmethylated C's in CHG context |
241126 |
Unmethylated C's in CHH context |
979823 |
Percentage methylation (CpG context) |
10.1% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot