Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_11_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_11_R2.fq.gz

Data processed at 23:10:07 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60961
Pairs without alignments under any condition 21931
Pairs that did not map uniquely 17108
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2144161
Methylated C's in CpG context 28507
Methylated C's in CHG context 1764
Methylated C's in CHH context 7966
Methylated C's in Unknown context 80
Unmethylated C's in CpG context 250610
Unmethylated C's in CHG context 374259
Unmethylated C's in CHH context 1481055
Unmethylated C's in Unknown context 5438
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15225 original top strand
CTOT 15162 complementary to original top strand
CTOB 15415 complementary to original bottom strand
OB 15158 original bottom strand

Deduplication

Alignments analysed 60960
Unique alignments 56886
Duplicates removed 4074
Duplicated alignments were found at 3795 different positions

Cytosine Methylation after Extraction

Total C's analysed 1358219
Methylated C's in CpG context 17603
Methylated C's in CHG context 1113
Methylated C's in CHH context 5036
Unmethylated C's in CpG context 157564
Unmethylated C's in CHG context 234678
Unmethylated C's in CHH context 942225
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot