Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_10_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_10_R2.fq.gz

Data processed at 23:10:07 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61154
Pairs without alignments under any condition 21890
Pairs that did not map uniquely 16956
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2154179
Methylated C's in CpG context 29648
Methylated C's in CHG context 1760
Methylated C's in CHH context 7608
Methylated C's in Unknown context 68
Unmethylated C's in CpG context 251436
Unmethylated C's in CHG context 375780
Unmethylated C's in CHH context 1487947
Unmethylated C's in Unknown context 5343
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15210 original top strand
CTOT 15214 complementary to original top strand
CTOB 15355 complementary to original bottom strand
OB 15374 original bottom strand

Deduplication

Alignments analysed 61153
Unique alignments 57147
Duplicates removed 4006
Duplicated alignments were found at 3715 different positions

Cytosine Methylation after Extraction

Total C's analysed 1367044
Methylated C's in CpG context 18292
Methylated C's in CHG context 1099
Methylated C's in CHH context 4850
Unmethylated C's in CpG context 158774
Unmethylated C's in CHG context 236251
Unmethylated C's in CHH context 947778
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot