Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61749 |
| Pairs without alignments under any condition |
21889 |
| Pairs that did not map uniquely |
16362 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2134624 |
| Methylated C's in CpG context |
28579 |
| Methylated C's in CHG context |
1697 |
| Methylated C's in CHH context |
7682 |
| Methylated C's in Unknown context |
75 |
| Unmethylated C's in CpG context |
246032 |
| Unmethylated C's in CHG context |
369826 |
| Unmethylated C's in CHH context |
1480808 |
| Unmethylated C's in Unknown context |
5344 |
| Percentage methylation (CpG context) |
10.4% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15222 |
original top strand |
| CTOT |
15485 |
complementary to original top strand |
| CTOB |
15661 |
complementary to original bottom strand |
| OB |
15381 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61749 |
| Unique alignments |
54631 |
| Duplicates removed |
7118 |
| Duplicated alignments were found at 6389 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1286222 |
| Methylated C's in CpG context |
16614 |
| Methylated C's in CHG context |
991 |
| Methylated C's in CHH context |
4536 |
| Unmethylated C's in CpG context |
147209 |
| Unmethylated C's in CHG context |
220589 |
| Unmethylated C's in CHH context |
896283 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.4% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot