Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3534_6_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3534_6_R2.fq.gz

Data processed at 23:10:07 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60946
Pairs without alignments under any condition 23409
Pairs that did not map uniquely 15645
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2131023
Methylated C's in CpG context 28664
Methylated C's in CHG context 1772
Methylated C's in CHH context 7458
Methylated C's in Unknown context 59
Unmethylated C's in CpG context 247062
Unmethylated C's in CHG context 369466
Unmethylated C's in CHH context 1476601
Unmethylated C's in Unknown context 5369
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15361 original top strand
CTOT 15232 complementary to original top strand
CTOB 15290 complementary to original bottom strand
OB 15063 original bottom strand

Deduplication

Alignments analysed 60946
Unique alignments 54560
Duplicates removed 6386
Duplicated alignments were found at 5727 different positions

Cytosine Methylation after Extraction

Total C's analysed 1329087
Methylated C's in CpG context 17277
Methylated C's in CHG context 1077
Methylated C's in CHH context 4605
Unmethylated C's in CpG context 152699
Unmethylated C's in CHG context 227992
Unmethylated C's in CHH context 925437
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot