Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61435 |
| Pairs without alignments under any condition |
22294 |
| Pairs that did not map uniquely |
16271 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2137438 |
| Methylated C's in CpG context |
31070 |
| Methylated C's in CHG context |
1884 |
| Methylated C's in CHH context |
8105 |
| Methylated C's in Unknown context |
60 |
| Unmethylated C's in CpG context |
244356 |
| Unmethylated C's in CHG context |
371466 |
| Unmethylated C's in CHH context |
1480557 |
| Unmethylated C's in Unknown context |
5408 |
| Percentage methylation (CpG context) |
11.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15396 |
original top strand |
| CTOT |
15226 |
complementary to original top strand |
| CTOB |
15288 |
complementary to original bottom strand |
| OB |
15525 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61435 |
| Unique alignments |
55086 |
| Duplicates removed |
6349 |
| Duplicated alignments were found at 5759 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1321450 |
| Methylated C's in CpG context |
18706 |
| Methylated C's in CHG context |
1142 |
| Methylated C's in CHH context |
4868 |
| Unmethylated C's in CpG context |
149852 |
| Unmethylated C's in CHG context |
227304 |
| Unmethylated C's in CHH context |
919578 |
| Percentage methylation (CpG context) |
11.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot