Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61752 |
| Pairs without alignments under any condition |
22130 |
| Pairs that did not map uniquely |
16118 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2153460 |
| Methylated C's in CpG context |
31225 |
| Methylated C's in CHG context |
1738 |
| Methylated C's in CHH context |
8146 |
| Methylated C's in Unknown context |
84 |
| Unmethylated C's in CpG context |
248966 |
| Unmethylated C's in CHG context |
375718 |
| Unmethylated C's in CHH context |
1487667 |
| Unmethylated C's in Unknown context |
5212 |
| Percentage methylation (CpG context) |
11.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15331 |
original top strand |
| CTOT |
15379 |
complementary to original top strand |
| CTOB |
15518 |
complementary to original bottom strand |
| OB |
15523 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61751 |
| Unique alignments |
55259 |
| Duplicates removed |
6492 |
| Duplicated alignments were found at 5909 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1334307 |
| Methylated C's in CpG context |
18547 |
| Methylated C's in CHG context |
1068 |
| Methylated C's in CHH context |
4983 |
| Unmethylated C's in CpG context |
153522 |
| Unmethylated C's in CHG context |
230168 |
| Unmethylated C's in CHH context |
926019 |
| Percentage methylation (CpG context) |
10.8% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot