Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3534_10_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3534_10_R2.fq.gz

Data processed at 23:10:06 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61864
Pairs without alignments under any condition 21587
Pairs that did not map uniquely 16549
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2178047
Methylated C's in CpG context 28747
Methylated C's in CHG context 1738
Methylated C's in CHH context 7062
Methylated C's in Unknown context 79
Unmethylated C's in CpG context 256943
Unmethylated C's in CHG context 382808
Unmethylated C's in CHH context 1500749
Unmethylated C's in Unknown context 5375
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15259 original top strand
CTOT 15471 complementary to original top strand
CTOB 15577 complementary to original bottom strand
OB 15557 original bottom strand

Deduplication

Alignments analysed 61864
Unique alignments 55456
Duplicates removed 6408
Duplicated alignments were found at 5806 different positions

Cytosine Methylation after Extraction

Total C's analysed 1340768
Methylated C's in CpG context 17015
Methylated C's in CHG context 1048
Methylated C's in CHH context 4335
Unmethylated C's in CpG context 157144
Unmethylated C's in CHG context 232950
Unmethylated C's in CHH context 928276
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%

M-Bias Plot