Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61864 |
| Pairs without alignments under any condition |
21587 |
| Pairs that did not map uniquely |
16549 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2178047 |
| Methylated C's in CpG context |
28747 |
| Methylated C's in CHG context |
1738 |
| Methylated C's in CHH context |
7062 |
| Methylated C's in Unknown context |
79 |
| Unmethylated C's in CpG context |
256943 |
| Unmethylated C's in CHG context |
382808 |
| Unmethylated C's in CHH context |
1500749 |
| Unmethylated C's in Unknown context |
5375 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15259 |
original top strand |
| CTOT |
15471 |
complementary to original top strand |
| CTOB |
15577 |
complementary to original bottom strand |
| OB |
15557 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61864 |
| Unique alignments |
55456 |
| Duplicates removed |
6408 |
| Duplicated alignments were found at 5806 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1340768 |
| Methylated C's in CpG context |
17015 |
| Methylated C's in CHG context |
1048 |
| Methylated C's in CHH context |
4335 |
| Unmethylated C's in CpG context |
157144 |
| Unmethylated C's in CHG context |
232950 |
| Unmethylated C's in CHH context |
928276 |
| Percentage methylation (CpG context) |
9.8% |
| Percentage methylation (CHG context) |
0.4% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot