Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
59448 |
| Pairs without alignments under any condition |
30289 |
| Pairs that did not map uniquely |
10263 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2600619 |
| Methylated C's in CpG context |
38599 |
| Methylated C's in CHG context |
5298 |
| Methylated C's in CHH context |
22801 |
| Methylated C's in Unknown context |
306 |
| Unmethylated C's in CpG context |
279475 |
| Unmethylated C's in CHG context |
425432 |
| Unmethylated C's in CHH context |
1829014 |
| Unmethylated C's in Unknown context |
7537 |
| Percentage methylation (CpG context) |
12.1% |
| Percentage methylation (CHG context) |
1.2% |
| Percentage methylation (CHH context) |
1.2% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
29633 |
original top strand |
| CTOT |
4 |
complementary to original top strand |
| CTOB |
9 |
complementary to original bottom strand |
| OB |
29802 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
59448 |
| Unique alignments |
57076 |
| Duplicates removed |
2372 |
| Duplicated alignments were found at 2305 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1980266 |
| Methylated C's in CpG context |
29502 |
| Methylated C's in CHG context |
4260 |
| Methylated C's in CHH context |
18399 |
| Unmethylated C's in CpG context |
213177 |
| Unmethylated C's in CHG context |
323900 |
| Unmethylated C's in CHH context |
1391028 |
| Percentage methylation (CpG context) |
12.2% |
| Percentage methylation (CHG context) |
1.3% |
| Percentage methylation (CHH context) |
1.3% |
|
|
M-Bias Plot