Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/5903_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/5903_R2.fq.gz

Data processed at 23:10:05 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 59448
Pairs without alignments under any condition 30289
Pairs that did not map uniquely 10263
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2600619
Methylated C's in CpG context 38599
Methylated C's in CHG context 5298
Methylated C's in CHH context 22801
Methylated C's in Unknown context 306
Unmethylated C's in CpG context 279475
Unmethylated C's in CHG context 425432
Unmethylated C's in CHH context 1829014
Unmethylated C's in Unknown context 7537
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29633 original top strand
CTOT 4 complementary to original top strand
CTOB 9 complementary to original bottom strand
OB 29802 original bottom strand

Deduplication

Alignments analysed 59448
Unique alignments 57076
Duplicates removed 2372
Duplicated alignments were found at 2305 different positions

Cytosine Methylation after Extraction

Total C's analysed 1980266
Methylated C's in CpG context 29502
Methylated C's in CHG context 4260
Methylated C's in CHH context 18399
Unmethylated C's in CpG context 213177
Unmethylated C's in CHG context 323900
Unmethylated C's in CHH context 1391028
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 1.3%

M-Bias Plot