Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
59788 |
| Pairs without alignments under any condition |
29986 |
| Pairs that did not map uniquely |
10226 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2557483 |
| Methylated C's in CpG context |
41376 |
| Methylated C's in CHG context |
3779 |
| Methylated C's in CHH context |
16013 |
| Methylated C's in Unknown context |
242 |
| Unmethylated C's in CpG context |
269479 |
| Unmethylated C's in CHG context |
418335 |
| Unmethylated C's in CHH context |
1808501 |
| Unmethylated C's in Unknown context |
7599 |
| Percentage methylation (CpG context) |
13.3% |
| Percentage methylation (CHG context) |
0.9% |
| Percentage methylation (CHH context) |
0.9% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
29983 |
original top strand |
| CTOT |
8 |
complementary to original top strand |
| CTOB |
10 |
complementary to original bottom strand |
| OB |
29787 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
59788 |
| Unique alignments |
56513 |
| Duplicates removed |
3275 |
| Duplicated alignments were found at 3170 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1891864 |
| Methylated C's in CpG context |
30700 |
| Methylated C's in CHG context |
3083 |
| Methylated C's in CHH context |
13000 |
| Unmethylated C's in CpG context |
199778 |
| Unmethylated C's in CHG context |
309087 |
| Unmethylated C's in CHH context |
1336216 |
| Percentage methylation (CpG context) |
13.3% |
| Percentage methylation (CHG context) |
1.0% |
| Percentage methylation (CHH context) |
1.0% |
|
|
M-Bias Plot