Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/5202_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/5202_R2.fq.gz

Data processed at 23:10:05 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60900
Pairs without alignments under any condition 28595
Pairs that did not map uniquely 10505
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2645668
Methylated C's in CpG context 37073
Methylated C's in CHG context 3678
Methylated C's in CHH context 15876
Methylated C's in Unknown context 262
Unmethylated C's in CpG context 282623
Unmethylated C's in CHG context 432596
Unmethylated C's in CHH context 1873822
Unmethylated C's in Unknown context 7796
Percentage methylation (CpG context) 11.6%
Percentage methylation (CHG context) 0.8%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 30633 original top strand
CTOT 6 complementary to original top strand
CTOB 6 complementary to original bottom strand
OB 30254 original bottom strand

Deduplication

Alignments analysed 60899
Unique alignments 57943
Duplicates removed 2956
Duplicated alignments were found at 2857 different positions

Cytosine Methylation after Extraction

Total C's analysed 1975314
Methylated C's in CpG context 28144
Methylated C's in CHG context 3074
Methylated C's in CHH context 13166
Unmethylated C's in CpG context 210593
Unmethylated C's in CHG context 323279
Unmethylated C's in CHH context 1397058
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 0.9%

M-Bias Plot