Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/3503_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/3503_R2.fq.gz

Data processed at 23:10:05 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 57332
Pairs without alignments under any condition 32794
Pairs that did not map uniquely 9874
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2445297
Methylated C's in CpG context 38255
Methylated C's in CHG context 3452
Methylated C's in CHH context 15550
Methylated C's in Unknown context 243
Unmethylated C's in CpG context 252250
Unmethylated C's in CHG context 395300
Unmethylated C's in CHH context 1740490
Unmethylated C's in Unknown context 7083
Percentage methylation (CpG context) 13.2%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 28576 original top strand
CTOT 6 complementary to original top strand
CTOB 8 complementary to original bottom strand
OB 28742 original bottom strand

Deduplication

Alignments analysed 57332
Unique alignments 54898
Duplicates removed 2434
Duplicated alignments were found at 2364 different positions

Cytosine Methylation after Extraction

Total C's analysed 1846095
Methylated C's in CpG context 29111
Methylated C's in CHG context 2883
Methylated C's in CHH context 12836
Unmethylated C's in CpG context 190000
Unmethylated C's in CHG context 297786
Unmethylated C's in CHH context 1313479
Percentage methylation (CpG context) 13.3%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.0%

M-Bias Plot