Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
61008 |
Pairs without alignments under any condition |
28396 |
Pairs that did not map uniquely |
10596 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2617130 |
Methylated C's in CpG context |
38787 |
Methylated C's in CHG context |
3814 |
Methylated C's in CHH context |
16132 |
Methylated C's in Unknown context |
318 |
Unmethylated C's in CpG context |
272956 |
Unmethylated C's in CHG context |
424987 |
Unmethylated C's in CHH context |
1860454 |
Unmethylated C's in Unknown context |
7826 |
Percentage methylation (CpG context) |
12.4% |
Percentage methylation (CHG context) |
0.9% |
Percentage methylation (CHH context) |
0.9% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
30455 |
original top strand |
CTOT |
5 |
complementary to original top strand |
CTOB |
9 |
complementary to original bottom strand |
OB |
30539 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
61008 |
Unique alignments |
57719 |
Duplicates removed |
3289 |
Duplicated alignments were found at 3173 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1914887 |
Methylated C's in CpG context |
28773 |
Methylated C's in CHG context |
3084 |
Methylated C's in CHH context |
12967 |
Unmethylated C's in CpG context |
199776 |
Unmethylated C's in CHG context |
310413 |
Unmethylated C's in CHH context |
1359874 |
Percentage methylation (CpG context) |
12.6% |
Percentage methylation (CHG context) |
1.0% |
Percentage methylation (CHH context) |
0.9% |
|
|
M-Bias Plot