Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/0503_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/0503_R2.fq.gz

Data processed at 23:10:04 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 59416
Pairs without alignments under any condition 30587
Pairs that did not map uniquely 9997
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2464248
Methylated C's in CpG context 40925
Methylated C's in CHG context 3621
Methylated C's in CHH context 17614
Methylated C's in Unknown context 284
Unmethylated C's in CpG context 249148
Unmethylated C's in CHG context 395141
Unmethylated C's in CHH context 1757799
Unmethylated C's in Unknown context 7377
Percentage methylation (CpG context) 14.1%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29711 original top strand
CTOT 9 complementary to original top strand
CTOB 20 complementary to original bottom strand
OB 29676 original bottom strand

Deduplication

Alignments analysed 59416
Unique alignments 56422
Duplicates removed 2994
Duplicated alignments were found at 2901 different positions

Cytosine Methylation after Extraction

Total C's analysed 1849861
Methylated C's in CpG context 31012
Methylated C's in CHG context 3012
Methylated C's in CHH context 14206
Unmethylated C's in CpG context 187357
Unmethylated C's in CHG context 296163
Unmethylated C's in CHH context 1318111
Percentage methylation (CpG context) 14.2%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.1%

M-Bias Plot