Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/0501_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/0501_R2.fq.gz

Data processed at 23:10:04 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 59640
Pairs without alignments under any condition 30009
Pairs that did not map uniquely 10351
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2510127
Methylated C's in CpG context 39770
Methylated C's in CHG context 3532
Methylated C's in CHH context 16591
Methylated C's in Unknown context 262
Unmethylated C's in CpG context 262307
Unmethylated C's in CHG context 406581
Unmethylated C's in CHH context 1781346
Unmethylated C's in Unknown context 7688
Percentage methylation (CpG context) 13.2%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29836 original top strand
CTOT 13 complementary to original top strand
CTOB 14 complementary to original bottom strand
OB 29777 original bottom strand

Deduplication

Alignments analysed 59640
Unique alignments 56557
Duplicates removed 3083
Duplicated alignments were found at 2972 different positions

Cytosine Methylation after Extraction

Total C's analysed 1859705
Methylated C's in CpG context 29965
Methylated C's in CHG context 2901
Methylated C's in CHH context 13262
Unmethylated C's in CpG context 193629
Unmethylated C's in CHG context 301308
Unmethylated C's in CHH context 1318640
Percentage methylation (CpG context) 13.4%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.0%

M-Bias Plot