Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
59640 |
| Pairs without alignments under any condition |
30009 |
| Pairs that did not map uniquely |
10351 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2510127 |
| Methylated C's in CpG context |
39770 |
| Methylated C's in CHG context |
3532 |
| Methylated C's in CHH context |
16591 |
| Methylated C's in Unknown context |
262 |
| Unmethylated C's in CpG context |
262307 |
| Unmethylated C's in CHG context |
406581 |
| Unmethylated C's in CHH context |
1781346 |
| Unmethylated C's in Unknown context |
7688 |
| Percentage methylation (CpG context) |
13.2% |
| Percentage methylation (CHG context) |
0.9% |
| Percentage methylation (CHH context) |
0.9% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
29836 |
original top strand |
| CTOT |
13 |
complementary to original top strand |
| CTOB |
14 |
complementary to original bottom strand |
| OB |
29777 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
59640 |
| Unique alignments |
56557 |
| Duplicates removed |
3083 |
| Duplicated alignments were found at 2972 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1859705 |
| Methylated C's in CpG context |
29965 |
| Methylated C's in CHG context |
2901 |
| Methylated C's in CHH context |
13262 |
| Unmethylated C's in CpG context |
193629 |
| Unmethylated C's in CHG context |
301308 |
| Unmethylated C's in CHH context |
1318640 |
| Percentage methylation (CpG context) |
13.4% |
| Percentage methylation (CHG context) |
1.0% |
| Percentage methylation (CHH context) |
1.0% |
|
|
M-Bias Plot