Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_8_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_8_R2.fastp.fq.gz

Data processed at 22:22:30 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61058
Pairs without alignments under any condition 21754
Pairs that did not map uniquely 17188
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2142676
Methylated C's in CpG context 29044
Methylated C's in CHG context 1764
Methylated C's in CHH context 8115
Methylated C's in Unknown context 64
Unmethylated C's in CpG context 247554
Unmethylated C's in CHG context 371803
Unmethylated C's in CHH context 1484396
Unmethylated C's in Unknown context 5274
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15358 original top strand
CTOT 15264 complementary to original top strand
CTOB 15255 complementary to original bottom strand
OB 15180 original bottom strand

Deduplication

Alignments analysed 61057
Unique alignments 57359
Duplicates removed 3698
Duplicated alignments were found at 3477 different positions

Cytosine Methylation after Extraction

Total C's analysed 1371764
Methylated C's in CpG context 17946
Methylated C's in CHG context 1112
Methylated C's in CHH context 5133
Unmethylated C's in CpG context 158229
Unmethylated C's in CHG context 236164
Unmethylated C's in CHH context 953180
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot