Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61058 |
| Pairs without alignments under any condition |
21754 |
| Pairs that did not map uniquely |
17188 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2142676 |
| Methylated C's in CpG context |
29044 |
| Methylated C's in CHG context |
1764 |
| Methylated C's in CHH context |
8115 |
| Methylated C's in Unknown context |
64 |
| Unmethylated C's in CpG context |
247554 |
| Unmethylated C's in CHG context |
371803 |
| Unmethylated C's in CHH context |
1484396 |
| Unmethylated C's in Unknown context |
5274 |
| Percentage methylation (CpG context) |
10.5% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15358 |
original top strand |
| CTOT |
15264 |
complementary to original top strand |
| CTOB |
15255 |
complementary to original bottom strand |
| OB |
15180 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61057 |
| Unique alignments |
57359 |
| Duplicates removed |
3698 |
| Duplicated alignments were found at 3477 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1371764 |
| Methylated C's in CpG context |
17946 |
| Methylated C's in CHG context |
1112 |
| Methylated C's in CHH context |
5133 |
| Unmethylated C's in CpG context |
158229 |
| Unmethylated C's in CHG context |
236164 |
| Unmethylated C's in CHH context |
953180 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot