Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_7_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_7_R2.fastp.fq.gz

Data processed at 22:22:30 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60610
Pairs without alignments under any condition 22770
Pairs that did not map uniquely 16620
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2136064
Methylated C's in CpG context 28501
Methylated C's in CHG context 1762
Methylated C's in CHH context 7409
Methylated C's in Unknown context 65
Unmethylated C's in CpG context 244939
Unmethylated C's in CHG context 366947
Unmethylated C's in CHH context 1486506
Unmethylated C's in Unknown context 5316
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15203 original top strand
CTOT 15012 complementary to original top strand
CTOB 15206 complementary to original bottom strand
OB 15189 original bottom strand

Deduplication

Alignments analysed 60610
Unique alignments 57111
Duplicates removed 3499
Duplicated alignments were found at 3310 different positions

Cytosine Methylation after Extraction

Total C's analysed 1384445
Methylated C's in CpG context 18084
Methylated C's in CHG context 1133
Methylated C's in CHH context 4741
Unmethylated C's in CpG context 158018
Unmethylated C's in CHG context 236329
Unmethylated C's in CHH context 966140
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot