Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
60610 |
| Pairs without alignments under any condition |
22770 |
| Pairs that did not map uniquely |
16620 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2136064 |
| Methylated C's in CpG context |
28501 |
| Methylated C's in CHG context |
1762 |
| Methylated C's in CHH context |
7409 |
| Methylated C's in Unknown context |
65 |
| Unmethylated C's in CpG context |
244939 |
| Unmethylated C's in CHG context |
366947 |
| Unmethylated C's in CHH context |
1486506 |
| Unmethylated C's in Unknown context |
5316 |
| Percentage methylation (CpG context) |
10.4% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15203 |
original top strand |
| CTOT |
15012 |
complementary to original top strand |
| CTOB |
15206 |
complementary to original bottom strand |
| OB |
15189 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
60610 |
| Unique alignments |
57111 |
| Duplicates removed |
3499 |
| Duplicated alignments were found at 3310 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1384445 |
| Methylated C's in CpG context |
18084 |
| Methylated C's in CHG context |
1133 |
| Methylated C's in CHH context |
4741 |
| Unmethylated C's in CpG context |
158018 |
| Unmethylated C's in CHG context |
236329 |
| Unmethylated C's in CHH context |
966140 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot