Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_6_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_6_R2.fastp.fq.gz

Data processed at 22:22:30 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61890
Pairs without alignments under any condition 21393
Pairs that did not map uniquely 16717
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2177048
Methylated C's in CpG context 28799
Methylated C's in CHG context 1808
Methylated C's in CHH context 7682
Methylated C's in Unknown context 44
Unmethylated C's in CpG context 250643
Unmethylated C's in CHG context 374718
Unmethylated C's in CHH context 1513398
Unmethylated C's in Unknown context 5279
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15418 original top strand
CTOT 15575 complementary to original top strand
CTOB 15425 complementary to original bottom strand
OB 15472 original bottom strand

Deduplication

Alignments analysed 61890
Unique alignments 57974
Duplicates removed 3916
Duplicated alignments were found at 3647 different positions

Cytosine Methylation after Extraction

Total C's analysed 1393231
Methylated C's in CpG context 17907
Methylated C's in CHG context 1145
Methylated C's in CHH context 4957
Unmethylated C's in CpG context 159814
Unmethylated C's in CHG context 237802
Unmethylated C's in CHH context 971606
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot