Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61890 |
| Pairs without alignments under any condition |
21393 |
| Pairs that did not map uniquely |
16717 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2177048 |
| Methylated C's in CpG context |
28799 |
| Methylated C's in CHG context |
1808 |
| Methylated C's in CHH context |
7682 |
| Methylated C's in Unknown context |
44 |
| Unmethylated C's in CpG context |
250643 |
| Unmethylated C's in CHG context |
374718 |
| Unmethylated C's in CHH context |
1513398 |
| Unmethylated C's in Unknown context |
5279 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15418 |
original top strand |
| CTOT |
15575 |
complementary to original top strand |
| CTOB |
15425 |
complementary to original bottom strand |
| OB |
15472 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61890 |
| Unique alignments |
57974 |
| Duplicates removed |
3916 |
| Duplicated alignments were found at 3647 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1393231 |
| Methylated C's in CpG context |
17907 |
| Methylated C's in CHG context |
1145 |
| Methylated C's in CHH context |
4957 |
| Unmethylated C's in CpG context |
159814 |
| Unmethylated C's in CHG context |
237802 |
| Unmethylated C's in CHH context |
971606 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot