Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_5_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_5_R2.fastp.fq.gz

Data processed at 22:22:30 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61443
Pairs without alignments under any condition 21828
Pairs that did not map uniquely 16729
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2175198
Methylated C's in CpG context 28268
Methylated C's in CHG context 1865
Methylated C's in CHH context 7941
Methylated C's in Unknown context 80
Unmethylated C's in CpG context 249908
Unmethylated C's in CHG context 375267
Unmethylated C's in CHH context 1511949
Unmethylated C's in Unknown context 5420
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15233 original top strand
CTOT 15534 complementary to original top strand
CTOB 15404 complementary to original bottom strand
OB 15271 original bottom strand

Deduplication

Alignments analysed 61442
Unique alignments 57597
Duplicates removed 3845
Duplicated alignments were found at 3605 different positions

Cytosine Methylation after Extraction

Total C's analysed 1398234
Methylated C's in CpG context 17568
Methylated C's in CHG context 1182
Methylated C's in CHH context 5075
Unmethylated C's in CpG context 159880
Unmethylated C's in CHG context 239225
Unmethylated C's in CHH context 975304
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot