Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61443 |
| Pairs without alignments under any condition |
21828 |
| Pairs that did not map uniquely |
16729 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2175198 |
| Methylated C's in CpG context |
28268 |
| Methylated C's in CHG context |
1865 |
| Methylated C's in CHH context |
7941 |
| Methylated C's in Unknown context |
80 |
| Unmethylated C's in CpG context |
249908 |
| Unmethylated C's in CHG context |
375267 |
| Unmethylated C's in CHH context |
1511949 |
| Unmethylated C's in Unknown context |
5420 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15233 |
original top strand |
| CTOT |
15534 |
complementary to original top strand |
| CTOB |
15404 |
complementary to original bottom strand |
| OB |
15271 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61442 |
| Unique alignments |
57597 |
| Duplicates removed |
3845 |
| Duplicated alignments were found at 3605 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1398234 |
| Methylated C's in CpG context |
17568 |
| Methylated C's in CHG context |
1182 |
| Methylated C's in CHH context |
5075 |
| Unmethylated C's in CpG context |
159880 |
| Unmethylated C's in CHG context |
239225 |
| Unmethylated C's in CHH context |
975304 |
| Percentage methylation (CpG context) |
9.9% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot